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Diagnostic Tests

The following diagnostic tests are performed on request at the D7 Anatomical Pathology laboratory, NHLS

ALCIAN BLUE for acid mucins

ALCIAN BLUE-PAS for acid- and neutral mucins

ALIZARIN RED S for calcium

AYOUB-SHLAR for keratin

BEST’S CARMINE for glycogen

BILE SIRIUS RED for bile and collagen

BISMARCK BROWN for mast cells

BROWN AND BRENN for Gram-positive and –negative bacilli

CONGO RED for Potassium permanganate

FITE’S MODIFIED ZIEHL-NEELSEN for mycobacteria, especially leprosy

GIEMSA – KIEL METHOD for inclusion bodies, toxoplasma and CMV

GOMORI’S METHENAMINE-SILVER for basement membrane

GOMORI’S ONE-STEP TRICHROME for connective tissue and muscle

GORDON AND SWEET’S for reticulin fibres

GRIMELIUS for argyrophil granules

GROCOTT’S GOMORI’S METHENAMINE SILVER for fungi

HAEMATOXYLIN AND EOSIN for nuclei and general tissue architecture

HALE’S DIALYZED (COLLOIDAL) IRON for acid mucins

HIGH IRON DIAMINE for sulphated- and sialo- acid mucins

HIGHMAN’S CONGO RED for amyloid

HYALURONIDASE for the removal of hyaluronic acid

LENDRUM’S PHLOXINE TARTRAZINE for viral inclusion bodies

LONG ZIEHL-NEELSEN for lipofuscin

LUXOL FAST BLUE – MODIFIED for myelin

MALLORY’S PHOSPHOTUNGSTIC ACID HAEM for neuroglial fibres

MARTIUS SCARLET BLUE for fibrin

MASSON FONTANA for argentaffin granules

MASSON FONTANA for melanin

MASSON’S TRICHROME for collagen and muscle

MELANIN BLEACH for removal of melanin

MILLER’S VICTORIA BLUE for elastic fibres

MODIFIED GIEMSA for Helicobacter pylori

OIL RED O for lipids

PALMGREN for nerve fibres

PERIODIC ACID SCHIFF’S for carbohydrates

PERIODIC ACID SCHIFF’S DIASTASE for removal of glycogen

PAS-OG for anterior pituitary cells

SOLOCHROME CYANINE for myelin

SOUTHGATE’S MUCICARMINE for mucin

SULPHATED ALCIAN BLUE (SAB) for amyloid

THIO-T for amyloid

UNNA PAP/ DALES METHYL GREEN-LIGHT-PYRONIN for RNA (plasma cells)

VAN GIESON for collagen

VERHOEFF’S ELASTIC VAN GIESON for elastic fibres

VON KOSSA for calcium deposits

WARTHIN STARRY for spirochaetes; cat scratch disease

ZIEHL-NEELSEN for acid fast bacilli